Biosystem Bts 310 User Manual
Total white blood cell (WBC) counts were performed by means of a manual method using blood diluted in 0.01% toluidine blue stain (Blue-Mclendon and Green, 2010Blue-Mclendon, A. and GREEN, RA., 2010. Hematology of ratites. In: D.J. WEISS and K.J. WARDROP, eds. Schalm's veterinary hematology. 6th ed. Ames: Lippincott Williams & Wilkins, pp. 987-993.) and the results were expressed in units per microliter (μL). Thin smears on glass slides were made immediately after the blood collection in order to avoid any interference with cell structures. Differential WBC counts, using an average of 200 cells, were made from blood films stained using Fast Panoptic method and were examined under an optical microscope with a 100x objective (in immersion oil).
biosystem bts 310 user manual
Total White Blood Cell (WBC) counts were performed by a manualmethod using blood diluted on 0.01% toluidine blue stain (Blue-McLendon andGreen, 2010) and the results were expressed in units per microliter([micro]L). Thin smears on glass slides were made immediately after the bloodcollection to avoid any interference on cell structure. Differential WBCcounts, using an average of 200 cells, were made from blood films stainedwith Fast Panoptic and examined under an optical microscope with a 100Xobjective (immersion oil). Images were captured using a digital camera (CanonPowerShot A640) with the aid of a computer and the Software Zeiss AxionVision. Erythrocytes, leukocytes and thrombocytes were identified andmorphologically characterized, and their length and width were measured(increase 100x). Five cells of each type were measured per blood films.